期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 134, 期 2, 页码 625-631出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2008.06.013
关键词
Amperometric immunosensor; Gold nanoparticles; Redox-active organic-inorganic composite film; Prussian blue; Carcinoembryonic antigen (CEA)
资金
- National Natural Science Foundation of China [20675064]
- Chinese Ministry Foundation for excellent young teachers
- Natural Science Foundation of Chongqing City [CSTC-2004BB4149, 2005BB4100]
- Foundation of Southwest University [XSGX 020]
A new highly sensitive amperometric immunosensor for the determination of carcinoembryonic antigen (CEA) had been constructed by employing a novel organic-inorganic composite film coupled with gold nanoparticles. At first, prussian blue (PB) was deposited on a glassy carbon electrode (GCE) and then a new porous organic material synthesized with 3,4,9,10-perylenetetracarboxylicdianhydride (PTCDA) and ethanediamine was coated on the surface of PB film to obtain a stable organic-inorganic composite film, which contained electrochemical redox activity and abundant amino groups on electrode interface. Furthermore, this porous organic material (PTC-NH2) could prevent the leakage of the PB efficiently which led to the significant enhancement of the stability and sensitivity of the immunosensor, as well as provided abundant amino-groups to assemble gold nanoparticles (nano-Au) for immobilization of carcinoembryonic antibody (anti-CEA). The morphologies of PB and the organic-inorganic composite films (PTC-NH2/PB) were studied by means of scanning electron microscope (SEM). In addition, the preparation procedure of the immunosensor was further investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under optimal conditions, the resulting immunosensor displayed a high sensitivity for the detection of CEA, and responded to the CEA concentration in two ranges from 0.05 to 2.0 ng/ml (R = 0.996) and from 2.0 to 40.0 ng/ml (R = 0.995) with a detection limit of 0.018 ng/ml. Moreover, the immunosensor exhibited high selectivity, long-term stability and good reproducibility. (c) 2008 Elsevier B.V. All rights reserved.
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