期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 129, 期 1, 页码 263-267出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2007.08.009
关键词
bioelectrocatalysis; substrate recycling; diaphorase; NADH oxidase; p-aminophenyl phosphate; electrochemical signal amplification
A novel strategy for the electrochemical signal amplification based on enzymatic recycling is presented. p-Aminophenyl phosphate was chosen as enzyme substrate and diaphorase and NADH oxidase as amplifying enzymes. Bioelectrocatalysis was observed by cyclic voltammetry using alkaline phosphatase as a model enzyme. Chronoamperometry experiments with diaphorase resulted in an amplification factor of 26.8. The amplification system was applied to the microcystin detection with a protein phosphatase 2A inhibition-based biosensor. The amplification enlarged the linear range by more than four orders of magnitude and decreased the limit of detection from 37.75 to 0.05 mu g/L, making the biosensor useful as a reliable screening tool to assess the quality of water. (c) 2007 Elsevier B.V. All rights reserved.
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