Micro patterned quantum dots excitation and imaging for cellular microarray screening

We developed a colloidal quantum dot (QD)-based multicolor excitation light source array designed for compact lab-on-a-chip cell screening and imaging. We have demonstrated multicolor ex vivo transmission mode microscopy to evaluate the nucleus-cytoplasm ratios of cancer cells. We have also performed immunofluorescence excitation of two types of cancer cells (MDA-MB 435 and SKBR3) that are cultured in a microwell array to quantify the disease specific protein expression. Printed array of color filters and microwells were used to perform fluorescence excitation and measurement of the biomarkers. Our method provides patterned multicolor light sources at low-cost that are suitable for high-throughput microarray cellular screening. (C) 2014 Elsevier B.V. All rights reserved.