期刊
SENSORS
卷 14, 期 8, 页码 14488-14499出版社
MDPI AG
DOI: 10.3390/s140814488
关键词
genotyping; suspended core optical fiber; molecular beacon; multiplexing; DNA detection
资金
- Australian Research Council (ARC)
- ARC Centre of Excellence in Nanoscale BioPhotonics
- Spinner International Mobility exchange fellowship
- ARC Georgina Sweet Laureate Fellowship
- ARC Future Fellowship
We report a novel approach to genotyping single nucleotide polymorphisms (SNPs) using molecular beacons in conjunction with a suspended core optical fiber (SCF). Target DNA sequences corresponding to the wild-or mutant-type have been accurately recognized by immobilizing two different molecular beacons on the core of a SCF. The two molecular beacons differ by one base in the loop-probe and utilize different fluorescent indicators. Single-color fluorescence enhancement was obtained when the immobilized SCFs were filled with a solution containing either wild-type or mutant-type sequence (homozygous sample), while filling the immobilized SCF with solution containing both wild-and mutant-type sequences resulted in dual-color fluorescence enhancement, indicating a heterozygous sample. The genotyping was realized amplification-free and with ultra low-volume for the required DNA solution (nano-liter). This is, to our knowledge, the first genotyping device based on the combination of optical fiber and molecular beacons.
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