4.6 Article

Genotyping Single Nucleotide Polymorphisms Using Different Molecular Beacon Multiplexed within a Suspended Core Optical Fiber

期刊

SENSORS
卷 14, 期 8, 页码 14488-14499

出版社

MDPI AG
DOI: 10.3390/s140814488

关键词

genotyping; suspended core optical fiber; molecular beacon; multiplexing; DNA detection

资金

  1. Australian Research Council (ARC)
  2. ARC Centre of Excellence in Nanoscale BioPhotonics
  3. Spinner International Mobility exchange fellowship
  4. ARC Georgina Sweet Laureate Fellowship
  5. ARC Future Fellowship

向作者/读者索取更多资源

We report a novel approach to genotyping single nucleotide polymorphisms (SNPs) using molecular beacons in conjunction with a suspended core optical fiber (SCF). Target DNA sequences corresponding to the wild-or mutant-type have been accurately recognized by immobilizing two different molecular beacons on the core of a SCF. The two molecular beacons differ by one base in the loop-probe and utilize different fluorescent indicators. Single-color fluorescence enhancement was obtained when the immobilized SCFs were filled with a solution containing either wild-type or mutant-type sequence (homozygous sample), while filling the immobilized SCF with solution containing both wild-and mutant-type sequences resulted in dual-color fluorescence enhancement, indicating a heterozygous sample. The genotyping was realized amplification-free and with ultra low-volume for the required DNA solution (nano-liter). This is, to our knowledge, the first genotyping device based on the combination of optical fiber and molecular beacons.

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