期刊
SENSORS
卷 14, 期 10, 页码 19329-19335出版社
MDPI AG
DOI: 10.3390/s141019329
关键词
DNA Origami; DNA Nanotechnology; Proton Detection; pH Sensors; i-motif; AFM; nanomechanical devices; Single-Molecule Sensors
资金
- Ministry of Education, Science, Sports, Culture and Technology, Japan [22220001, 24350088, 24104004]
- PRESTO, JST
- Kansai University
- Grants-in-Aid for Scientific Research [25107734, 22220001, 24104004] Funding Source: KAKEN
Single-molecule pH sensors have been developed by utilizing molecular imaging of pH-responsive shape transition of nanomechanical DNA origami devices with atomic force microscopy (AFM). Short DNA fragments that can form i-motifs were introduced to nanomechanical DNA origami devices with pliers-like shape (DNA Origami Pliers), which consist of two levers of 170-nm long and 20-nm wide connected at a Holliday-junction fulcrum. DNA Origami Pliers can be observed as in three distinct forms; cross, antiparallel and parallel forms, and cross form is the dominant species when no additional interaction is introduced to DNA Origami Pliers. Introduction of nine pairs of 12-mer sequence (5'-AACCCCAACCCC-3'), which dimerize into i-motif quadruplexes upon protonation of cytosine, drives transition of DNA Origami Pliers from open cross form into closed parallel form under acidic conditions. Such pH-dependent transition was clearly imaged on mica in molecular resolution by AFM, showing potential application of the system to single-molecular pH sensors.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据