期刊
SENSORS
卷 13, 期 5, 页码 5777-5795出版社
MDPI
DOI: 10.3390/s130505777
关键词
synthetic biology; scaffolds; genetic circuits
资金
- NSF [CBET1129012]
Whole-cell biosensors are a good alternative to enzyme-based biosensors since they offer the benefits of low cost and improved stability. In recent years, live cells have been employed as biosensors for a wide range of targets. In this review, we will focus on the use of microorganisms that are genetically modified with the desirable outputs in order to improve the biosensor performance. Different methodologies based on genetic/protein engineering and synthetic biology to construct microorganisms with the required signal outputs, sensitivity, and selectivity will be discussed.
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