期刊
SENSORS
卷 12, 期 6, 页码 6967-6977出版社
MDPI AG
DOI: 10.3390/s120606967
关键词
microwell; single-cell; electric lysis; electroosmotic-driven; microfluidics
资金
- National Science Council of Taiwan [NSC 99-2923-E-194-001-MY3]
- NSC [100-2113-M-006-007-MY2]
Accurate analysis at the single-cell level has become a highly attractive tool for investigating cellular content. An electroosmotic-driven microfluidic chip with arrays of 30-mu m-diameter microwells was developed for single-cell electric lysis in the present study. The cellular occupancy in the microwells when the applied voltage was 5 V (82.4%) was slightly higher than that at an applied voltage of 10 V (81.8%). When the applied voltage was increased to 15 V, the cellular occupancy in the microwells dropped to 64.3%. More than 50% of the occupied microwells contain individual cells. The results of electric lysis experiments at the single-cell level indicate that the cells were gradually lysed as the DC voltage of 30 V was applied; the cell was fully lysed after 25 s. Single-cell electric lysis was demonstrated in the proposed microfluidic chip, which is suitable for high-throughput cell lysis.
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