期刊
SENSORS
卷 11, 期 11, 页码 10388-10397出版社
MDPI
DOI: 10.3390/s111110388
关键词
sensor; glucose; chemiluminescence; DNAzyme; quantum dots; hydrogen peroxide; G-quadruplex; chemiluminescence resonance energy transfer
资金
- EU
A hybrid consisting of glucose oxidase-functionalized with hemin/G-quadruplex units is used for the chemiluminescence detection of glucose. The glucose oxidase-mediated oxidation of glucose yields gluconic acid and H2O2. The latter in the presence of luminol acts as substrate for the hemin/G-quadruplex-catalyzed generation of chemiluminescence. The glucose oxidase/hemin G-quadruplex hybrid was immobilized on CdSe/ZnS quantum dots (QDs). The light generated by the hybrid, in the presence of glucose, activated a chemiluminescence resonance energy transfer process to the QDs, resulting in the luminescence of the QDs. The intensities of the luminescence of the QDs at different concentrations of glucose provided an optical means to detect glucose.
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