期刊
SENSORS
卷 8, 期 2, 页码 1004-1024出版社
MDPI
DOI: 10.3390/s8021004
关键词
chemical labeling; protein; fluorescent imaging; tag; metal-chelation
Site-specific chemical labeling utilizing small fluorescent molecules is a powerful and attractive technique for in vivo and in vitro analysis of cellular proteins, which can circumvent some problems in genetic encoding labeling by large fluorescent proteins. In particular, affinity labeling based on metal-chelation, advantageous due to the high selectivity/simplicity and the small tag-size, is promising, as well as enzymatic covalent labeling, thereby a variety of novel methods have been studied in recent years. This review describes the advances in chemical labeling of proteins, especially highlighting the metal-chelation methodology.
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