期刊
SENSORS
卷 8, 期 9, 页码 5775-5791出版社
MDPI
DOI: 10.3390/s8095775
关键词
screen-printer biosensor; alkaline phosphatase; Fe3O4 nanoparticles; 2,4-dichlorophenoxyacetic acid
资金
- Malaysian Ministry of Science, Technology and Innovation (MOSTI) [09-03-03-0184-EA001, 09-03-03-0006 BIOTEK]
- Universiti Kebangsaan Malaysia [OUP2008]
Magnetic nanoparticles of Fe3O4 were synthesized and characterized using transmission electron microscopy and X-ray diffraction. The Fe3O4 nanoparticles were found to have an average diameter of 5.48 +/- 1.37 nm. An electrochemical biosensor based on immobilized alkaline phosphatase ( ALP) and Fe3O4 nanoparticles was studied. The amperometric biosensor was based on the reaction of ALP with the substrate ascorbic acid 2-phosphate (AA2P). The incorporation of the Fe3O4 nanoparticles together with ALP into a sol gel/chitosan biosensor membrane has led to the enhancement of the biosensor response, with an improved linear response range to the substrate AA2P (5-120 mu M) and increased sensitivity. Using the inhibition property of the ALP, the biosensor was applied to the determination of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). The use of Fe3O4 nanoparticles gives a two-fold improvement in the sensitivity towards 2,4-D, with a linear response range of 0.5-30 mu gL(-1). Exposure of the biosensor to other toxicants such as heavy metals demonstrated only slight interference from metals such as Hg2+, Cu2+, Ag2+ and Pb2+. The biosensor was shown to be useful for the determination of the herbicide 2, 4-D because good recovery of 95-100 percent was obtained, even though the analysis was performed in water samples with a complex matrix. Furthermore, the results from the analysis of 2,4-D in water samples using the biosensor correlated well with a HPLC method.
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