期刊
SENSOR LETTERS
卷 9, 期 6, 页码 2320-2326出版社
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/sl.2011.1809
关键词
Zeolite; Nanobiocomposite; Urease; FET Biosensor; Urea
资金
- European Union [PIRSES-GA-2008-230802]
- NATO [CBP.NUKR.CLG 984221]
Zeolite/enzyme nanobiocomposites of different compositions were tested in this work for the improvement of biosensor analytical characteristics. The bioselective element based on urease immobilized by cross-linking with glutaraldehyde was used as a model. The working characteristics of biosensors based on various zeolite/enzyme nanocomposites were examined and compared with those of urease-based biosensors. An optimal concentration of zeolytes beta (BEA) in bioselective elements is determined to be 1.5%. It ensures as wide linear range of measurement without remarkable loss in biosensor sensitivity to urea. The BEA zeolite-based biosensors were shown to have better working parameters in comparison with those based on zeolites A (LTA). A decrease in biosensor sensitivity to heavy metal ions was demonstrated for all zeolites used, which testifies to probable increase in stability of urea measurement in real environmental samples.
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