期刊
SCIENTIFIC REPORTS
卷 5, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/srep18478
关键词
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资金
- Ministry of Education, Culture, Sports, Science and Technology [KAKENHI 26248043]
- Ministry of Economy, Trade, and Industry
- Japan Science and Technology Agency (JST) Precursory Research for Embryonic Science and Technology (PRESTO)
- intramural research program of the NIH, National Cancer Institute
We present a remodeling method for high-affinity unnatural-base DNA aptamers to augment their thermal stability and nuclease resistance, for use as drug candidates targeting specific proteins. Introducing a unique mini-hairpin DNA provides robust stability to unnatural-base DNA aptamers generated by SELEX using genetic alphabet expansion, without reducing their high affinity. By this method, >80% of the remodeled DNA aptamer targeting interferon-gamma (K-D of 33 pM) survived in human serum at 37 degrees C after 3 days under our experimental conditions, and sustainably inhibited the biological activity of interferon-gamma.
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