MCM10: One tool for all-Integrity, maintenance and damage control

Minichromsome maintenance protein 10 (Mcm10) is an essential replication factor that is required for the activation of the Cdc45:Mcm2-7: GINS helicase. Mcm10's ability to bind both ds and ssDNA appears vital for this function. In addition, Mcm10 interacts with multiple players at the replication fork, including DNA polymerase-a and proliferating cell nuclear antigen with which it cooperates during DNA elongation. Mcml 0 lacks enzymatic function, but instead provides the replication apparatus with an oligomeric scaffold that likely acts in the coordination of DNA unwinding and DNA synthesis. Not surprisingly, loss of Mcm10 engages checkpoint, DNA repair and SUMO-dependent rescue pathways that collectively counteract replication stress and chromosome breakage. Here, we review Mcml O's structure and function and explain how it contributes to the maintenance of genome integrity. (C) 2014 Elsevier Ltd. All rights reserved.