期刊
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
卷 24, 期 8-9, 页码 661-667出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.semcdb.2013.05.001
关键词
Stable isotope; Quantitative imaging; Stem cell; Human; Metabolism; Cell division
资金
- NIA NIH HHS [R21 AG034641] Funding Source: Medline
- NIBIB NIH HHS [P41 EB001974] Funding Source: Medline
- NIDDK NIH HHS [K08 DK090147] Funding Source: Medline
Multi-isotope imaging mass spectrometry (MIMS) is the quantitative imaging of stable isotope labels in cells with a new type of secondary ion mass spectrometer (NanoSIMS). The power of the methodology is attributable to (i) the immense advantage of using non-toxic stable isotope labels, (ii) high resolution imaging that approaches the resolution of usual transmission electron microscopy and (iii) the precise quantification of label down to 1 part-per-million and spanning several orders of magnitude. Here we review the basic elements of MIMS and describe new applications of MIMS to the quantitative study of metabolic processes including protein and nucleic acid synthesis in model organisms ranging from microbes to humans. (C) 2013 Published by Elsevier Ltd.
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