期刊
SCIENTIFIC REPORTS
卷 5, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/srep10473
关键词
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资金
- National Basic Research Program of China ('973' Program) [2012CB720600, 2013CB933800]
- National Natural Science Foundation of China [21422201, 21372018]
- Innovation Team of Ministry of Education [BMU 20110263]
DNA cross-linking technology is an attractive tool for the detection, regulation, and manipulation of genes. In this study, a series of photolabile4-oxo-enal-modified oligonucleotides functionalized with photosensitive O-nitrobenzyl derivatives were rationally designed as a new kind of photocaged crosslinking agents. A comprehensive evaluation of cross-linking reactions for different nucleobases in complementary strands under different conditions suggested that the modified DNA oligonucleotides tended to form interstrand cross-linking to nucleobases with the potential of thymidine > guanosine >> cytidine similar to adenosine. Different from previous literature reports that cytidine and adenosine were preferential cross-linked nucleobases with 4-oxo-enal moieties, our study represents the first example of DNA cross-linking for T and G selectivity using 4-oxo-enal moiety. The cross-linked adducts were identified and their cross-linking mechanism was also illustrated. This greatly expands the applications of 4-oxo-enal derivatives in the studies of DNA damage and RNA structure
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