期刊
SCIENTIA HORTICULTURAE
卷 127, 期 3, 页码 388-397出版社
ELSEVIER
DOI: 10.1016/j.scienta.2010.09.024
关键词
Orchid; Expressed sequence tags; Suppression subtractive hybridization
类别
资金
- Ministry of Science, Technology and Innovation (MOSTI)
- Universiti Putra Malaysia [01-02-04-0474 PR0035/04-01, 05-04-08-0551 RU]
A subtracted cDNA library of open flower was constructed using the suppression subtractive hybridization (SSH) technique, to identify fragrance-related transcripts of Vanda Mimi Palmer. In total, 107 transcripts up-regulated during blooming were identified and sequenced. Only 33 clones (4 singletons and 29 contigs) showed similarities to known sequences in the public database. Of these, thirty-two clones were transcripts encoding fragrance-related enzymes including sesquiterpene synthase,(+/-)-germacrene D synthase, tyrosine decarboxylase and putative acyltransferase. Two fragrance-related transcripts, VMPAAT encoding a putative alcohol acyltransferase and VMPSTS encoding a sesquiterpene synthase, were subjected to full-length cDNA isolation and characterization. The full length cDNA of VMPAAT has a 1343 bp open reading frame (ORF) of 448 amino acid residues whereas VMPSTS is predicted to encode a polypeptide of 561 amino acid residues with 1682 bp ORF. VMPAAT and VMPSTS show high homologies with plant alcohol acyltransferase and terpene synthase, respectively. Real time RT-PCR indicated that both transcripts were expressed preferentially in floral tissues, with high levels in blooming and full bloom flowers. VMPAAT and VMPSTS transcripts were expressed in a rhythmic pattern. The results presented in this study will be potentially useful in providing additional insights into the fragrance-related pathways of Orchidaceae members, which until today is still limited. (C) 2010 Elsevier B.V. All rights reserved.
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