期刊
SCIENTIA HORTICULTURAE
卷 125, 期 2, 页码 110-116出版社
ELSEVIER
DOI: 10.1016/j.scienta.2010.02.001
关键词
Satsuma mandarin; Flavonoid; Chalcone synthase; Chalcone isomerase; Real-time PCR; HPLC
类别
资金
- National Public Benefit (Agriculture) Research Foundation [nyhyzx07-023]
- Ministry of Science and Technology [2004EP090019]
Chalcone synthase (CHS) and chalcone isomerase (CHI) are two key genes involved in flavonoid biosynthesis. They were both cloned from Guoqing No. 4 satsuma mandarin (Citrus unshiu marcow), and their relative expression and accumulation of corresponding flavonoid components during fruit maturation were investigated by real-time PCR and HPLC techniques, respectively. During fruit maturation, expression of CHS and CHI genes declined gradually in peels, as well as the concentrations of total flavonoids, trans-chalcone, narirutin and hesperidin; in pulps, however, expression of both genes showed an approximately uptrend, and the concentrations of total flavonoids and those three components were detected in a lower level without significant changes among different developmental periods (P < 0.05). This research confirmed that expression of CHS and CHI genes was positively correlated with flavonoid accumulation and overexpression of them could be a potential approach to produce massive desired flavonoids in citrus fruits. (C) 2010 Published by Elsevier B.V.
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