期刊
SCIENTIA HORTICULTURAE
卷 121, 期 4, 页码 419-424出版社
ELSEVIER
DOI: 10.1016/j.scienta.2009.03.012
关键词
Embryogenesis; Microspore culture; Heat shock; Purple flowering stalk
类别
资金
- National High Science and Technology Program (863) [2008AA10Z155]
- Science and Technology Innovation Foundation of Huazhong Agricultural University [2008XCX014]
Purple flowering stalk (Brassica campestris ssp. chinensis var. purpurea Hort.) was generally regarded as a recalcitrant species for microspore culture in Brassica crops. Conditions for reliable induction of microspore embryogenesis were studied in 12 genotypes of purple flowering stalk. A treatment of short heat shock to microspore by incubating at 32 degrees C for 18 h was suitable for the survival of microspores, sustained cell divisions, and further induced embryogenesis. Subsequently, the reduced concentration of macro salts (1/2 NLN) provided an optimal condition for the development of embryoids. Under the optimized conditions for microspore embryoid development, 10 genotypes responded to microspore culture with the frequencies ranging from 2.7 to 70.5 embryoids per dish. However, regenerated plants were obtained from 9 genotypes, and more than 75% these regenerated plants were double haploid. This report establishes an efficient protocol for microspore culture and offers great potential for DH breeding in purple flowering stalk. (c) 2009 Elsevier B.V. All rights reserved.
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