期刊
SCIENTIA HORTICULTURAE
卷 120, 期 2, 页码 237-245出版社
ELSEVIER
DOI: 10.1016/j.scienta.2008.11.015
关键词
Cultivar identification; Genetic relationship; Peach-based rootstocks; Plums; SSR markers
类别
资金
- CICYT (Comision Interministerial de Ciencia y Tecnologia) [AGL 2005-05533]
- INIA (Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria) [RF03-014-C2, RF2007-00026-C02-01]
- DGA (Diputacion General de Aragon) [A44]
- Spanish Ministry of Foreign Affairs
- CSIC-FSE [I3P-PC2006]
Twenty microsatellite primer pairs, previously developed in peach, were used to characterize and to explore genetic relationships among 44 clones, representing three groups of rootstocks defined as: (1) Peach-based rootstocks (Prunus dulcis x P. persica, P. persica x P. davidiana); (2) Myrobalan-Marianna plums (P. cerasifera and interspecific hybrids having A cerasifera as a parent); and (3) Slow growing plums (P. insititia, P. domestica, and P. domestica x P. spinosa). Eighteen SSR markers, from the 20 initially used, were able to amplify polymorphic products for the Peach-based rootstocks and 13 common markers gave also polymorphism for the Myrobalan-Marianna and Slow growing plums groups. The Dice coefficient of similarity was calculated between all pairs of accessions and their genetic similarity represented by a principal coordinate analysis. The genetic diversity detected among the 44 clones studied divided them in three groups, which are in agreement with their current taxonomic classification and their morphological characteristics. A set of three microsatellites (BPPCT001, CPPCT022 and UDP98-407) can distinguish between all the clones analyzed. The analysis within groups reveal another two sets of three SSR to distinguish between the clones from the Peach based rootstocks and the Myrobalan-Marianna plums, respectively, and only a single SSR is needed to distinguish within the clones from the Slow growing plums group. These results demonstrate the high potential of the SSR analysis for peach rootstock identification and studies of diversity in Prunus species. (C) 2008 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据