期刊
SCIENTIA HORTICULTURAE
卷 118, 期 1, 页码 33-38出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scienta.2008.05.017
关键词
plantlet conversion; Psidium guajava; shoot tips; short-term storage; synthetic seeds
类别
资金
- Council of Scientific & Industrial Research (CSIR), New Delhi
Shoot tips obtained from in vitro grown plantlets of guava (Psidium guajava L) were encapsulated in calcium alginate beads for short-term storage and germplasm exchange. A gelling matrix of 3% sodium alginate and 100 mM calcium chloride was found most suitable for formation of ideal calcium alginate beads. Maximum percent response for conversion of encapsulated shoot tips into plantlets was obtained on growth regulator free full strength liquid MS medium. The regrowth ability of encapsulated shoot tips was affected by medium strength and sucrose concentrations in the medium. Encapsulated shoot tips could be stored at low temperature (4 degrees C) up to 30 days with a survival frequency of 25%. After 60 days of storage under minimal growth conditions (sucrose lacking medium), about 75% encapsulated shoot tips were converted into plantlets when subcultured on 3% sucrose containing medium. Plantlets regenerated from encapsulated shoot tips were acclimatized successfully. (c) 2008 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据