Impaired formation of homotypic cell-in-cell structures in human tumor cells lacking alpha-catenin expression

Although cell-in-cell structures (CICs) could be detected in a wide range of human tumors, homotypic CICs formed between tumor cells occur at low rate for most of them. We recently reported that tumor cells lacking expression of E-and P-cadherin were incapable of forming homotypic CICs by entosis, and re-expression of E-or P-cadherin was sufficient to induce CICs formation in these tumor cells. In this work, we found that homotypic CICs formation was impaired in some tumor cells expressing high level of E-cadherin due to loss expression of alpha-catenin (a-catenin), a molecular linker between cadherin-mediated adherens junctions and F-actin. Expression of a-catenin in these tumor cells restored cell-cell adhesion and promoted CICs formation in a ROCK kinase-dependent way. Thus, our work identified a-catenin as another molecule in addition to E-and P-cadherin that were targeted to inactivate homotypic CICs formation in human tumor cells.