期刊
SCIENCE SIGNALING
卷 1, 期 12, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/stke.112pt2
关键词
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资金
- NIGMS NIH HHS [T32 GM007753] Funding Source: Medline
The rules by which naive T cells decide whether and how to respond to antigenic stimuli are incompletely understood. Using multiphoton intravital microscopy (MP-IVM) in lymphnodes (LNs), we have shown that CD8(+) T cells are primed by antigen-presenting dendritic cells (DCs) in three consecutive phases. During phaseone, T cells undergo brief serial contacts with many DCs for several hours after homing in to the LNs. Subsequently, during phase two, T cells engage in prolonged stable interactions with DCs. Finally, in the third phase, T cells return to transient interactions with DCs as they begin to proliferate and eventually leave the LNs. We have examined the influence of antigen dose on the duration of phase one by systematically varying both the number of cognate peptide-major his to compatability (pMHC) complexes per DC and the density of cognate pMHC complex-presenting DCs per LN. The duration of phase one and the kinetics of CD8+ T cell activation were inversely correlated with both parameters. Very few pMHC complexes were needed for full T cell activation and effect or differentiation. Further more, there was a sharp threshold of antigen dose below which T cells did not transition to phase two but continued to migrate until they exited the LN, unactivated. The stability of peptide binding to MHC was a critical determinant of this threshold antigen dose in vivo. Our results suggest an integrative mechanism that allows T cells to reach an informed decision about whether to respond, based on the over all antigen dose encountered.
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