期刊
SCIENCE OF THE TOTAL ENVIRONMENT
卷 470, 期 -, 页码 1408-1413出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scitotenv.2013.07.037
关键词
Urinary phthalate metabolites; Gas chromatography-mass spectrometry; Trimethylsilylation; Isotope dilution; Biomonitoring
Phthalates are used in industry products, household items, and medical tools as plasticizers. Human exposure to phthalates has raised concern about its toxicity. In the present study, optimization was conducted for the simultaneous analysis of eight kinds of phthalate metabolites using gas chromatography-mass spectrometry (GC-MS): MEP, MiBP, MnBP, MBzP, MiNP, MEHP, MEOHP, and MEHHP. In order to minimize the matrix effect and to do quantitative analysis, isotope dilution and LLE-GC-MS methods were performed. Urine samples were enzymatically hydrolyzed, extracted with a mixture of n-hexane and ethyl ether (8:2; v:v), and subsequently derivatized with trimethylsilylation. All eight kinds of analytes showed clear resolution and high reproducibility in GC-MS results. The method detection limit ranged from 0.05 ng/mL to 0.2 ng/mL. Calibration curves were found to be linear from 0.2 to 100 ng/mL with -(2) > 0.992. The relative standard deviation of the intraday precision using water and urine ranged from 2.1% to 163%. The analysis was performed with urine samples that were collected from adults residing in the Republic of Korea. The analyzed concentration results were compared according to gender and region. As a result, DEHP metabolites showed the highest detected concentration (75.92 mu g/g creatinine, 100%), and MiNP, a metabolite of DiNP, showed the lowest detected concentration (0.42 mu g/g creatinine, 22.5%). On average, female urine (200.76 mu g/g creatinine) had a higher detected concentration of Sigma(8) phthalate metabolites than male urine. Samples from rural regions (211.96 mu g/g creatinine) had higher levels than samples from urban regions. (C) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据