期刊
SCIENCE OF THE TOTAL ENVIRONMENT
卷 468, 期 -, 页码 813-820出版社
ELSEVIER
DOI: 10.1016/j.scitotenv.2013.08.100
关键词
Antibiotic resistance gene; Conjugation; Tetracycline; Sulfamethoxazole; Activated sludge
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology [2012-0003505]
- Korea Ministry of Environment as Global Top Project [GT-11-B-01-005-1]
The presence of antibiotics in the natural environment has been a growing issue. This presence could also account for the influence that affects microorganisms in such a way that they develop resistance against these antibiotics. The aim of this study was to evaluate whether the antibiotic resistant gene (ARC) plasrnid transfer can be facilitated by the impact of 1) environmentally representative micro-contaminant concentrations in ppb (part per billion) levels and 2) donor-recipient microbial complexity (pure vs. mixed). For this purpose, the multidrug resistant plasmid, pB10, and Escherichia coli DH5 alpha were used as a model plasmid and a model donor, respectively. Based on conjugation experiments with pure (Pseudomonas aeruginosa PAKexoT) and mixed (activated sludge) cultures as recipients, increased relative plasmid transfer frequencies were observed at ppb (mu g/L) levels of tetracycline and sulfamethoxazole micro-contaminant exposure. When sludge, a more complex community, was used as a recipient, the increases of the plasmid transfer rate were always statistically significant but not always in P. aeruginosa. The low concentration (10 ppb) of tetracycline exposure led to the pB10 transfer to enteric bacteria, which are clinically important pathogens. (C) 2013 Elsevier B.V. All rights reserved.
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