Effect of acanthocephalan infection on metal, total protein and metallothionein concentrations in European chub from a Sava River section with low metal contamination

In the present study, the importance of considering fish intestinal parasites i.e. the acanthocephalans in metal exposure assessment was estimated under low metal contamination conditions. Two acanthocephalan species, Pomphorhynchus laevis and Acanthocephalus anguillae were examined in 59 specimens of European chub (Squalius cephalus L.) sampled at 5 locations along the Sava River, Croatia. Concentrations of essential (Cu, Mn) and non-essential (Ag, Cd, Pb) metals were higher in intestinal parasites than chub gastrointestinal tissue, but levels of essential metals Fe and Zn were comparable or lower in parasites, respectively. The highest accumulation in both acanthocephalan species was found for non-essential metals and followed the order: Ag > Pb > Cd. Higher infection intensity with P. laevis allowed us to present their spatial metal distribution and evaluate the influence of P. laevis on metal levels and sub-cellular biological responses (total protein and metallothionein levels) in the host infected with P. laevis. Even in the river section with low metal contamination, parasitism affected metal levels, resulting in lower Cu, Cd and Pb concentrations in chub infected with P. laevis than in uninfected chub. Although total protein and metallothionein levels remained constant in infected and uninfected chub, acanthocephalans should be considered a potential confounding factor in metal exposure assessments. Moreover, P. laevis-chub system can be suggested as an appropriate tool in biomonitoring, since in both species increased Cu and Cd concentrations towards the downstream locations were found. Higher Cu and Cd levels in P. laevis suggest acanthocephalans to be sensitive bioindicators if low metal levels have to be detected. (C) 2013 Elsevier B.V. All rights reserved.