期刊
SCIENCE OF THE TOTAL ENVIRONMENT
卷 414, 期 -, 页码 664-671出版社
ELSEVIER
DOI: 10.1016/j.scitotenv.2011.11.045
关键词
Vermicomposting; Bioactive organic material; Eisenia fetida; Bacterial community; 16S RNA gene; Pathogenic bacteria
资金
- Junta de Andalucia [P05-AGR-00408]
- Marie Curie Actions [MEIF-CT-2006-041034]
- Science and Innovation Ministry [AP2006-03452]
This study describes the use of denaturing gradient gel electrophoresis (DGGE) and COMPOCHIP (i.e. a microarray targeting typical bacteria of stabilized organic materials and pathogenic bacteria) for investigating the bacterial communities of four different vermicomposts. These included a commercial vermicompost produced from cattle manure (CM) and three vermicomposts produced at pilot-scale by recycling: damaged tomato fruits (DT); olive-mill waste mixed with biosolids (OB); and winery wastes (WW). DGGE provided distinctive fingerprints of each vermicompost, which were statistically related to their particular chemical features. The comparison of the various vermicompost fingerprints showed that they contained bacterial communities with an average similarity coefficient of close to 80%. COMPOCHIP detected the presence of Sphingobacterium, Streptomyces, Alpha-Proteobacteria, Delta-Proteobacteria, and Firmicutes in all the vermicomposts. COMPOCHIP showed differences in the abundance of particular bacterial taxa among the vermicomposts, giving an idea about the usefulness of each vermicompost in the search for bacteria valuable to biotechnology. The joint use of DGGE and COMPOCHIP is a useful tool to compare vermicompost bacterial communities and to assess the potential of different vermicomposts as bioactive organic materials. (C) 2011 Elsevier B.V. All rights reserved.
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