期刊
SCIENCE OF THE TOTAL ENVIRONMENT
卷 408, 期 7, 页码 1501-1507出版社
ELSEVIER
DOI: 10.1016/j.scitotenv.2009.12.017
关键词
Copper (II) resistant bacteria; Biosorption; Bioreduction; Copper bioleaching from soil; Vineyard soil and copper-mining waste
资金
- Brazilian National Research Council (CNPq)
- Auburn University
Copper is an essential but toxic heavy metal that negatively impacts living systems at high concentration. This study presents factors affecting copper bioremoval (bioreduction and biosorption) by a highly copper resistant monoculture of Pseudomonas sp. NA and copper bioremoval from soil. Seven bacteria resistant to high concentration of Cu(II) were isolated from enrichment cultures of vineyard soils and mining wastes. Culture parameters influencing copper bioreduction and biosorption by one monoculture isolate were studied. The isolate was identified by 16S rRNA gene sequence analysis as a Pseudomonas sp. NA (98% similarity to Pseudomonas putida, Pseudomonas plecoglossicida and other Pseudomonas sp.). The optimal temperature for growth was 30 degrees C and bioremoval of Cu(II) was maximal at 35 degrees C. Considerable growth of the isolate was observed between pH 5.0 and 8.0 with the highest growth and biosorption recorded at pH 6.0. Maximal bioreduction was observed at pH 5.0. Cu(II) bioremoval was directly proportional to Cu(II) concentration in media. Pseudomonas sp. NA removed more than 110 mg L-1 Cu(II) in water within 24 h through bioreduction and biosorption at initial concentration of 300 mg L-1. In cultures amended with 100 mg L-1. 20.7 mg L-1 of Cu(II) was biologically reduced and more than 23 mg L-1 of Cu(II) was biologically removed in 12 h. The isolate strongly promoted copper bioleaching in soil. Results indicate that Pseudomonas sp. NA has good potential as an agent for removing copper from water and soil. Published by Elsevier B.V.
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