期刊
SCIENCE OF THE TOTAL ENVIRONMENT
卷 407, 期 13, 页码 4184-4188出版社
ELSEVIER
DOI: 10.1016/j.scitotenv.2009.01.042
关键词
Fluorescence spectroscopy; Transmission electron microscopy; Toxicity evaluation
资金
- Natural Science Foundation of China [20607011, 20875055, NCET-06-0582]
- Ministry of Education of China [708058]
- Foundation for Excellent Young Scientists and Key Science-Technology Project in Shandong Province [2007BS08005, 2008GG10006012]
Measuring protein damage by nanomaterials may give insight into the mechanisms of toxicity of nanomaterials. The toxic effects of nanoAg on bovine serum albumin (BSA) were thoroughly studied using fluorescence spectroscopy, ultraviolet-visible absorption spectroscopy, resonance light scattering spectroscopy (RLS). circular dichroism spectroscopy (CD) and transmission electron microscopy (TEM). NanoAg had obvious toxic effects on BSA: nanoAg could increase the amount of helix and decrease the beta sheet structure, leading to a loosening of the protein skeleton. In the loose structure, internal hydrophobic amino acids are exposed and the characteristic fluorescence of BSA is obviously quenched. When the ratio of nanoAg and BSA increased to 1: 96 (wt/wt), the impact of nanoAg on the spectral properties leveled off. The RLS spectrum, TEM, CD spectra and electrophoresis results showed that BSA had destroyed the double-layer structure of nanoAg and covered its surface, generating a BSA-nanoAg complex held together by van der Waals and electrostatic forces. This paper provides a new perspective and method for determining the toxic effects of nanoAg on biological macromolecules. (C) 2009 Elsevier B.V. All rights reserved.
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