期刊
SCIENCE OF THE TOTAL ENVIRONMENT
卷 402, 期 1, 页码 29-35出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scitotenv.2008.04.005
关键词
uranium; amphibians; embryo and larvae acute toxicity assays; heavy metals; Rana perezi Seoane
Amphibians have been reported as sensitive organisms whose survival has been impaired by several environmental factors. Nevertheless, sometimes amphibians are found inhabiting extreme environments. Thus, in order to perceive how Iberian green frogs (Rana perezi Seoane) are able to survive in the ponds of a uranium mine (Central Portugal) this study aimed to assess the ecotoxicological effects promoted by the mine effluent in the early-life stages of this species. To attain this objective, eggs (collected in a nearby reference river) and laboratory hatching larvae were exposed during 96 h to different dilutions of the effluent. All the effects on the hatch success were recorded. The highest concentration of the effluent produced a significant decrease in body length of larvae, as well as a decrease in stimulus reactions and an increase in pigmentation along with tail deformities. A recovery assay showed an increased bicaccumulation of metals, uranium included, resulting from increased effluent exposure. (c) 2008 Elsevier B.V. All rights reserved.
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