期刊
SCIENCE CHINA-LIFE SCIENCES
卷 56, 期 7, 页码 619-627出版社
SCIENCE PRESS
DOI: 10.1007/s11427-013-4504-2
关键词
genome mining; streptothricin; Streptomyces; heterologous expression; biosynthesis
类别
资金
- National Natural Science Foundation of China [31170037]
- Ministry of Science and Technology of China [2013CB734003]
- China Postdoctoral Science Foundation [2013M530755]
Streptothricins (STs) are used commercially to treat bacterial and fungal diseases in agriculture. Mining of the sequenced microbial genomes uncovered two cryptic ST clusters from Streptomyces sp. C and Streptomyces sp. TP-A0356. The ST cluster from S. sp. TP-A0356 was verified by successful heterologous expression in Streptomyces coelicolor M145. Two new ST analogs were produced together with streptothricin F and streptothricin D in the heterologous host. The ST cluster was further confirmed by inactivation of gene stnO, which was proposed encoding an aminomutase supplying beta-lysines for the poly-beta-Lys chain formation. A putative biosynthetic pathway for STs is proposed based on bioinformatics analyses of the ST genes and experimental evidence.
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