期刊
SCIENCE CHINA-CHEMISTRY
卷 55, 期 4, 页码 508-514出版社
SCIENCE PRESS
DOI: 10.1007/s11426-012-4543-8
关键词
microfluidic chip; isothermal amplification; lung cancer; confocal detection; non-linear filter
资金
- National Supporting Plan of China [2011BAZ03288, 2012BAI23B00]
- National Program on Key Basic Research Projects of China [2011CB707701]
- National Natural Science Foundation of China [60978064]
- Science Plan Foundation of Zhejiang [2012C31G3210006]
- TSINGHUA-SIEMENS Collective Foundation [20103000273]
This work presents a promising clinical molecular diagnostics for early stage lung cancer. This novel diagnostic method utilized the loop-mediated isothermal amplification (LAMP), microfluidic chips and a confocal optical detector with a non-linear fluorescent filter processor. An isothermal amplification based microfluidic chip for the early diagnostics of lung cancer was developed and a confocal optical detector was improved by a novel real-time fluorescent filter to sensitively monitor the DNA amplification procedure with high signal to noise ratio and fluorescence collecting ability. Experiment showed that a rapid diagnostic of lung cancer by detecting the existence of the CEA mRNA could be performed in only 5 mu L of reaction assay in less than 45 min. While the traditional in-tube RT-PCR set consumed more than 25 mu L of the assay and took more than 90 min.
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