Determination of abscisic acid based on the fluorescent quenching of quantum dots

The quenching mechanism of the fluorescence of quantum dots by abscisic acid has been systematically investigated. The quenching constant K-SV = 5.1 x 10(11)/M was obtained under the optimized condition. On the basis of that, a very sensitive method for the determination of abscisic acid has been developed. The linear equation was F-0/F = 0.9309 + 0.5072 C (pmol/L) and its linear range was 0.2-3.0 pmol/L with a correlation coefficient of 0.9939. The limit of detection was 0.09 pmol/L.