期刊
SCIENCE CHINA-CHEMISTRY
卷 53, 期 1, 页码 52-60出版社
SCIENCE PRESS
DOI: 10.1007/s11426-010-0011-5
关键词
Ca2+; EF-hand calcium binding pockets; protein grafting approach; Streptococcus pyrogenes; Sindbis virus
资金
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R01EB007268] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM062999, R01GM081749] Funding Source: NIH RePORTER
- NIBIB NIH HHS [R01 EB007268-03, R01 EB007268] Funding Source: Medline
- NIGMS NIH HHS [R01 GM081749-04, R01 GM081749, R01 GM062999-04, R01 GM062999] Funding Source: Medline
Ca2+ plays a pivotal role in the physiology and biochemistry of prokaryotic and mammalian organisms. Viruses also utilize the universal Ca2+ signal to create a specific cellular environment to achieve coexistence with the host, and to propagate. In this paper we first describe our development of a grafting approach to understand site-specific Ca2+ binding properties of EF-hand proteins with a helix-loop-helix Ca2+ binding motif, then summarize our prediction and identification of EF-hand Ca2+ binding sites on a genome-wide scale in bacteria and virus, and next report the application of the grafting approach to probe the metal binding capability of predicted EF-hand motifs within the streptococcal hemoprotein receptor (Shr) of Streptococcus pyrogenes and the nonstructural protein 1 (nsP1) of Sindbis virus. When methods such as the grafting approach are developed in conjunction with prediction algorithms we are better able to probe continuous Ca2+-binding sites that have been previously underrepresented due to the limitation of conventional methodology.
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