期刊
SCIENCE CHINA-CHEMISTRY
卷 53, 期 4, 页码 812-819出版社
SCIENCE PRESS
DOI: 10.1007/s11426-009-0168-y
关键词
micro-plate magnetic chemiluminescence immunoassay; carcinoembryonic antigen; tumor marker
资金
- CAS [YZ-0632]
- National Natural Science Founolation of China [20907060]
- CAS Graduate Innovation Foundation [YXLW-3]
- National key Technology RD Program [2006BAF07B03-1-1]
- Shaanxi Province, China's Scholarship Council [08JK482]
A micro-plate magnetic chemiluminescence immunoassay was developed for rapid and high throughput detection of carcinoembryonic antigens (CEA) in human sera. This method was based on a sandwich immunoreaction of fluorescein isothiocyanate (FITC)-labeled anti-CEA antibodies, CEA antigens, and horseradish peroxidase (HRP)-conjugated anti-CEA antibodies in micro-plate. The immunomagnetic particles coated with anti-FITC antibodies were used as the solid phase for the immunoassay. The separation procedure was carried out by a magnetic plate adaptor and the luminol-hydrogen peroxide (H2O2)-HRP system was employed for the chemiluminescence detection. The proposed method combined the advantages of the micro-plate reactor and magnetic particle separation technology with the linear range of 5-250 ng mL(-1). The detection limit of CEA was 0.61 ng mL(-1). The coefficient of the variation was less than 7% and 13% for intra-assay and inter-assay precision, respectively. Compared with the commercial micro-plate chemiluminescent kit, the proposed method showed a good correlation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据