期刊
SCIENCE
卷 362, 期 6411, 页码 240-242出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aau5174
关键词
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资金
- University of California
- San Francisco Program for Breakthrough in Biomedical Research
- Sandler Foundation
- National Institutes of Health (NIH) Office of the Director Early Independence Award [DP5-OD021344]
- DARPA Safe Genes grant [HR0011-17-2-0043]
- Desmond and Ann Heathwood MGH Research Scholar Award
- NIH [K99 CA218870, R35 GM118158]
Bacterial CRISPR-Cas systems protect their host from bacteriophages and other mobile genetic elements. Mobile elements, in turn, encode various anti-CRISPR (Acr) proteins to inhibit the immune function of CRISPR-Cas. To date, Acr proteins have been discovered for type I (subtypes I-D, I-E, and I-F) and type II (II-A and II-C) but not other CRISPR systems. Here, we report the discovery of 12 acr genes, including inhibitors of type V-A and I-C CRISPR systems. AcrVA1 inhibits a broad spectrum of Cas12a (Cpf1) orthologs-including MbCas12a, Mb3Cas12a, AsCas12a, and LbCas12a-when assayed in human cells. The acr genes reported here provide useful biotechnological tools and mark the discovery of acr loci in many bacteria and phages.
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