期刊
SCIENCE
卷 345, 期 6199, 页码 940-943出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1255083
关键词
-
资金
- European Research Council [233348]
- ANR [BACNET 09-BLAN-0024-02]
- ANR Investissement d'Avenir Programme [10-LABX-62]
- Fondation Le Roch
- Fondation Jeantet
Riboswitches are ligand-binding elements contained within the 5' untranslated regions of bacterial transcripts, which generally regulate expression of downstream open reading frames. Here, we show that in Listeria monocytogenes, a riboswitch that binds vitamin B-12 controls expression of a noncoding regulatory RNA, Rli55. Rli55, in turn, controls expression of the eut genes, whose products enable ethanolamine utilization and require B12 as a cofactor. Defects in ethanolamine utilization, or in its regulation by Rli55, significantly attenuate Listeria virulence in mice. Rli55 functions by sequestering the two-component response regulator EutV by means of a EutV-binding site contained within the RNA. Thus, Rli55 is a riboswitch-regulated member of the small group of regulatory RNAs that function by sequestering a protein and reveals a distinctive mechanism of signal integration in bacterial gene regulation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据