期刊
SCIENCE
卷 346, 期 6213, 页码 1077-+出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1258096
关键词
-
资金
- Alexander von Humboldt Foundation
- German Federal Ministry for Education and Research
- Helmholtz Association
- German Research Foundation
- Goran Gustafsson Foundation
- Swedish Research Council
- Kempe Foundation
- Umea University
- Howard Hughes Medical Institute
- NSF
- Gates Foundation
- Li Ka Shing Foundation
- NIH
The advent of facile genome engineering using the bacterial RNA-guided CRISPR-Cas9 system in animals and plants is transforming biology. We review the history of CRISPR (clustered regularly interspaced palindromic repeat) biology from its initial discovery through the elucidation of the CRISPR-Cas9 enzyme mechanism, which has set the stage for remarkable developments using this technology to modify, regulate, or mark genomic loci in a wide variety of cells and organisms from all three domains of life. These results highlight a new era in which genomic manipulation is no longer a bottleneck to experiments, paving the way toward fundamental discoveries in biology, with applications in all branches of biotechnology, as well as strategies for human therapeutics.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据