期刊
SCIENCE
卷 344, 期 6185, 页码 742-746出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1248167
关键词
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资金
- BBSRC [BB/G021392/1]
- BHF [PG/08/011/24416, PG/10/43/28390]
- EPSRC
- MRC [G0500707]
- National Institute of Health Research
- BBSRC [BB/G021392/1] Funding Source: UKRI
- MRC [G0500707] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/G021392/1] Funding Source: researchfish
- British Heart Foundation [RG/09/003/27122, PG/10/43/28390, RG/11/14/29056] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [1235152] Funding Source: researchfish
- Medical Research Council [G0500707] Funding Source: researchfish
Nuclear magnetic resonance (NMR) spectroscopy is useful to determine molecular structure in tissues grown in vitro only if their fidelity, relative to native tissue, can be established. Here, we use multidimensional NMR spectra of animal and in vitro model tissues as fingerprints of their respective molecular structures, allowing us to compare the intact tissues at atomic length scales. To obtain spectra from animal tissues, we developed a heavy mouse enriched by about 20% in the NMR-active isotopes carbon-13 and nitrogen-15. The resulting spectra allowed us to refine an in vitro model of developing bone and to probe its detailed structure. The identification of an unexpected molecule, poly(adenosine diphosphate ribose), that may be implicated in calcification of the bone matrix, illustrates the analytical power of this approach.
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