期刊
SCIENCE
卷 346, 期 6216, 页码 1533-1536出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1255301
关键词
-
资金
- NIH [DP1 OD000217, R01 GM085286, 1 U54 CA143869]
- Fondation Pierre Gilles de Gennes
- Donna and Benjamin M. Rosen Center for Bioengineering at Caltech
Variability in gene expression among genetically identical cells has emerged as a central preoccupation in the study of gene regulation; however, a divide exists between the predictions of molecular models of prokaryotic transcriptional regulation and genome-wide experimental studies suggesting that this variability is indifferent to the underlying regulatory architecture. We constructed a set of promoters in Escherichia coli in which promoter strength, transcription factor binding strength, and transcription factor copy numbers are systematically varied, and used messenger RNA (mRNA) fluorescence in situ hybridization to observe how these changes affected variability in gene expression. Our parameter-free models predicted the observed variability; hence, the molecular details of transcription dictate variability in mRNA expression, and transcriptional noise is specifically tunable and thus represents an evolutionarily accessible phenotypic parameter.
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