期刊
SCIENCE
卷 346, 期 6208, 页码 439-+出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1257998
关键词
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资金
- Howard Hughes Medical Institute (HHMI)
- Janelia Visitor Program
- NIH [R01GM080370, RM01-GM61010, R01HD37047, GM033830]
- Division of Intramural Research, National Heart, Lung, and Blood Institute, NIH
- Japan Society for the Promotion of Science-NEXT program [LS128]
- Uehara Memorial Foundation
- Takeda Science Foundation
- Kurata Memorial Hitachi Science and Technology Foundation
- RIKEN Center for Developmental Biology
- HHMI
- Human Frontier Science Program [LT000926/2012]
- European Research Council [281903]
- Grants-in-Aid for Scientific Research [25650071] Funding Source: KAKEN
- European Research Council (ERC) [281903] Funding Source: European Research Council (ERC)
Although fluorescence microscopy provides a crucial window into the physiology of living specimens, many biological processes are too fragile, are too small, or occur too rapidly to see clearly with existing tools. We crafted ultrathin light sheets from two-dimensional optical lattices that allowed us to image three-dimensional (3D) dynamics for hundreds of volumes, often at subsecond intervals, at the diffraction limit and beyond. We applied this to systems spanning four orders of magnitude in space and time, including the diffusion of single transcription factor molecules in stem cell spheroids, the dynamic instability of mitotic microtubules, the immunological synapse, neutrophil motility in a 3D matrix, and embryogenesis in Caenorhabditis elegans and Drosophila melanogaster. The results provide a visceral reminder of the beauty and the complexity of living systems.
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