期刊
SCIENCE
卷 340, 期 6132, 页码 619-621出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1234848
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资金
- Wellcome Trust
- UK Biotechnology and Biological Sciences Research Council (BBSRC)
- European Research Council
- BBSRC [BBS/E/J/000CA369] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/J/000CA369] Funding Source: researchfish
Roles for long noncoding RNAs (lncRNAs) in gene expression are emerging, but regulation of the lncRNA itself is poorly understood. We have identified a homeodomain protein, AtNDX, that regulates COOLAIR, a set of antisense transcripts originating from the 3' end of Arabidopsis FLOWERING LOCUS C (FLC). AtNDX associates with single-stranded DNA rather than double-stranded DNA non-sequence-specifically in vitro, and localizes to a heterochromatic region in the COOLAIR promoter in vivo. Single-stranded DNA was detected in vivo as part of an RNA-DNA hybrid, or R-loop, that covers the COOLAIR promoter. R-loop stabilization mediated by AtNDX inhibits COOLAIR transcription, which in turn modifies FLC expression. Differential stabilization of R-loops could be a general mechanism influencing gene expression in many organisms.
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