期刊
SCIENCE
卷 341, 期 6144, 页码 379-383出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1236231
关键词
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资金
- NIH from the National Center for Research Resources [P41RR005351]
- National Institute of General Medical Sciences [P41GM103390, R01GM090269]
- Institute of Allergy and Infectious Disease [AI058113]
- Centers for Disease Control
- Netherlands Organization for Scientific Research (NWO)
A systematic, efficient means of producing diverse libraries of asymmetrically branched N-glycans is needed to investigate the specificities and biology of glycan-binding proteins. To that end, we describe a core pentasaccharide that at potential branching positions is modified by orthogonal protecting groups to allow selective attachment of specific saccharide moieties by chemical glycosylation. The appendages were selected so that the antenna of the resulting deprotected compounds could be selectively extended by glycosyltransferases to give libraries of asymmetrical multi-antennary glycans. The power of the methodology was demonstrated by the preparation of a series of complex oligosaccharides that were printed as microarrays and screened for binding to lectins and influenza-virus hemagglutinins, which showed that recognition is modulated by presentation of minimal epitopes in the context of complex N-glycans.
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