期刊
SCIENCE
卷 341, 期 6149, 页码 1009-1012出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1240985
关键词
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资金
- DGF [LO1627/1-1]
- European Research Council (ERC) [310343]
- European Molecular Biology Organization
- Agence Nationale de la Recherche [ANR-11-BSV8-016]
- Ministere de l'Enseignement et de la Recherche [ED387]
- University of Basel
- Swiss National Science Foundation [31003A_132428/1]
- Austrian Science Fund [J3148-B12]
- International Max Planck Research School for Molecular and Cellular Life Sciences (IMPRS)
- FEBS long-term fellowship
- Swiss National Science Foundation (SNF) [31003A_132428] Funding Source: Swiss National Science Foundation (SNF)
- European Research Council (ERC) [310343] Funding Source: European Research Council (ERC)
Intraflagellar transport (IFT) of ciliary precursors such as tubulin from the cytoplasm to the ciliary tip is involved in the construction of the cilium, a hairlike organelle found on most eukaryotic cells. However, the molecular mechanisms of IFT are poorly understood. Here, we found that the two core IFT proteins IFT74 and IFT81 form a tubulin-binding module and mapped the interaction to a calponin homology domain of IFT81 and a highly basic domain in IFT74. Knockdown of IFT81 and rescue experiments with point mutants showed that tubulin binding by IFT81 was required for ciliogenesis in human cells.
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