期刊
SCIENCE
卷 342, 期 6156, 页码 357-360出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1241459
关键词
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资金
- U.S. Department of Energy [DE-FG02-02ER63445]
- NSF [SA5283-11210]
- NIH [NIDDK-K01DK089006, 1DP5OD009172-01]
- Defense Advanced Research Projects Agency [N66001-12-C-4040, N66001-12-C-4020, N66001-12-C-4211]
- Arnold and Mabel Beckman Foundation
- U.S. Department of Defense National Defense Science and Engineering Graduate Fellowship
- NSF
- Assistant Secretary of Defense for Research and Engineering [FA8721-05-C-0002]
- [NIH-MSTP-TG-T32GM07205]
We describe the construction and characterization of a genomically recoded organism (GRO). We replaced all known UAG stop codons in Escherichia coli MG1655 with synonymous UAA codons, which permitted the deletion of release factor 1 and reassignment of UAG translation function. This GRO exhibited improved properties for incorporation of nonstandard amino acids that expand the chemical diversity of proteins in vivo. The GRO also exhibited increased resistance to T7 bacteriophage, demonstrating that new genetic codes could enable increased viral resistance.
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