期刊
SCIENCE
卷 338, 期 6109, 页码 932-936出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1225624
关键词
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资金
- Deutsche Forschungsgemeinschaft [SFB 863]
- Bundesministerium fur Bildung und Forschung [13N10970]
- European Research Council [GA 256270]
- Technische Universitat Munchen Institute for Advanced Study
- NIH [1R01GM081705]
We created nanometer-scale transmembrane channels in lipid bilayers by means of self-assembled DNA-based nanostructures. Scaffolded DNA origami was used to create a stem that penetrated and spanned a lipid membrane, as well as a barrel-shaped cap that adhered to the membrane, in part via 26 cholesterol moieties. In single-channel electrophysiological measurements, we found similarities to the response of natural ion channels, such as conductances on the order of 1 nanosiemens and channel gating. More pronounced gating was seen for mutations in which a single DNA strand of the stem protruded into the channel. Single-molecule translocation experiments show that the synthetic channels can be used to discriminate single DNA molecules.
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