期刊
SCIENCE
卷 337, 期 6098, 页码 1101-1104出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1216861
关键词
-
资金
- NIH
- NSF
- U.S. Department of Energy [2R01GM057089-13, NSF GK-12 742551, DE-SC0004917, DE-FG02-09ER25917]
- U.S. Department of Energy (DOE) [DE-SC0004917] Funding Source: U.S. Department of Energy (DOE)
Enzymes are thought to have evolved highly specific catalytic activities from promiscuous ancestral proteins. By analyzing a genome-scale model of Escherichia coli metabolism, we found that 37% of its enzymes act on a variety of substrates and catalyze 65% of the known metabolic reactions. However, it is not apparent why these generalist enzymes remain. Here, we show that there are marked differences between generalist enzymes and specialist enzymes, known to catalyze a single chemical reaction on one particular substrate in vivo. Specialist enzymes (i) are frequently essential, (ii) maintain higher metabolic flux, and (iii) require more regulation of enzyme activity to control metabolic flux in dynamic environments than do generalist enzymes. Furthermore, these properties are conserved in Archaea and Eukarya. Thus, the metabolic network context and environmental conditions influence enzyme evolution toward high specificity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据