期刊
SCIENCE
卷 336, 期 6088, 页码 1581-1584出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1221976
关键词
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资金
- National Institutes of Health [3P01GM072694-05S1]
- German Research Foundation [RA 1781/1-1, SFB 803]
- Fonds der Chemischen Industrie [684052, 166660, 661218]
- National Commission for Scientific and Technological Research of Chile
Cellular membrane fusion is thought to proceed through intermediates including docking of apposed lipid bilayers, merging of proximal leaflets to form a hemifusion diaphragm, and fusion pore opening. A membrane-bridging four-helix complex of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) mediates fusion. However, how assembly of the SNARE complex generates docking and other fusion intermediates is unknown. Using a cell-free reaction, we identified intermediates visually and then arrested the SNARE fusion machinery when fusion was about to begin. Partial and directional assembly of SNAREs tightly docked bilayers, but efficient fusion and an extended form of hemifusion required assembly beyond the core complex to the membrane-connecting linkers. We propose that straining of lipids at the edges of an extended docking zone initiates fusion.
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