期刊
SCIENCE
卷 338, 期 6107, 页码 671-675出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1224350
关键词
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资金
- Wellcome Trust
- NIH
- Deutsche Forschungsgemeinschaft
- European Molecular Biology Laboratory
- Swiss National Fonds
- European Molecular Biology Organization
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [0747197] Funding Source: National Science Foundation
- Cancer Research UK [16358] Funding Source: researchfish
Eukaryotic genomes are extensively transcribed, forming both messenger RNAs (mRNAs) and noncoding RNAs (ncRNAs). ncRNAs made by RNA polymerase II often initiate from bidirectional promoters (nucleosome-depleted chromatin) that synthesize mRNA and ncRNA in opposite directions. We demonstrate that, by adopting a gene-loop conformation, actively transcribed mRNA encoding genes restrict divergent transcription of ncRNAs. Because gene-loop formation depends on a protein factor (Ssu72) that coassociates with both the promoter and the terminator, the inactivation of Ssu72 leads to increased synthesis of promoter-associated divergent ncRNAs, referred to as Ssu72-restricted transcripts (SRTs). Similarly, inactivation of individual gene loops by gene mutation enhances SRT synthesis. We demonstrate that gene-loop conformation enforces transcriptional directionality on otherwise bidirectional promoters.
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