期刊
SCIENCE
卷 336, 期 6089, 页码 1704-1708出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1220757
关键词
-
资金
- GlaxoSmithKline Australia
- Garfield Weston Foundation for the Centre for Novel Agricultural Products
- Biotechnology and Biological Sciences Research Council [1098027] Funding Source: researchfish
Noscapine is an antitumor alkaloid from opium poppy that binds tubulin, arrests metaphase, and induces apoptosis in dividing human cells. Elucidation of the biosynthetic pathway will enable improvement in the commercial production of noscapine and related bioactive molecules. Transcriptomic analysis revealed the exclusive expression of 10 genes encoding five distinct enzyme classes in a high noscapine-producing poppy variety, HN1. Analysis of an F-2 mapping population indicated that these genes are tightly linked in HN1, and bacterial artificial chromosome sequencing confirmed that they exist as a complex gene cluster for plant alkaloids. Virus-induced gene silencing resulted in accumulation of pathway intermediates, allowing gene function to be linked to noscapine synthesis and a novel biosynthetic pathway to be proposed.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据