期刊
SCIENCE
卷 336, 期 6083, 页码 934-937出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1220671
关键词
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资金
- Biotechnology and Biological Sciences Research Council (BBSRC)
- Medical Research Council
- Wellcome Trust
- European Union EpiGeneSys
- BLUEPRINT
- Cancer Research UK
- MRC [G0801156] Funding Source: UKRI
- Medical Research Council [G0801156] Funding Source: researchfish
5-Methylcytosine can be converted to 5-hydroxymethylcytosine (5hmC) in mammalian DNA by the ten-eleven translocation (TET) enzymes. We introduce oxidative bisulfite sequencing (oxBS-Seq), the first method for quantitative mapping of 5hmC in genomic DNA at single-nucleotide resolution. Selective chemical oxidation of 5hmC to 5-formylcytosine (5fC) enables bisulfite conversion of 5fC to uracil. We demonstrate the utility of oxBS-Seq to map and quantify 5hmC at CpG islands (CGIs) in mouse embryonic stem (ES) cells and identify 800 5hmC-containing CGIs that have on average 3.3% hydroxymethylation. High levels of 5hmC were found in CGIs associated with transcriptional regulators and in long interspersed nuclear elements, suggesting that these regions might undergo epigenetic reprogramming in ES cells. Our results open new questions on 5hmC dynamics and sequence-specific targeting by TETs.
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